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Image Search Results
Journal: Scientific Reports
Article Title: Pregnane X receptor (PXR) as a prognostic marker and therapeutic target in kidney renal clear cell carcinoma (KIRC) via AMPK pathway regulation
doi: 10.1038/s41598-025-19812-y
Figure Lengend Snippet: PXR regulates KIRC cell proliferation via the AMPK pathway. ( A ) Representative bright-field images showing distinct proliferation states of 786-O cells in the NC group, siRNA group, and siRNA + AMPKi group (Total Magnification: 40X); ( B ) CCK-8 proliferation assay was performed to evaluate cell growth capacity over a 36-h time course; ( C ) Western blot analysis depicting the expression profiles of key proteins (PXR, p-AMPK, AMPK, PI3K, p-PI3K, AKT, p-AKT, p53, Bax, Bcl-2, PCNA, Cyclin D1) in the NC, siRNA, and siRNA + AMPKi groups; β-actin served as the loading control; ( D ) Quantitative analysis of protein expression levels from panel C, normalized to β-actin; ( E ) Immunofluorescence staining illustrating the subcellular localization and expression of p-AMPK (green) and PXR (red) proteins in the three groups; nuclei were counterstained with DAPI (blue) (Total Magnification: 200X); scale bar: 20 μm. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 vs. NC group, # p < 0.05, ## p < 0.01 vs. siRNA group.
Article Snippet: The primary antibodies used included: PXR (67912-1-Ig, Proteintech, Wuhan, China), p-AMPK (AP1441, Abclonal, Wuhan, China), AMPK (A1229, Abclonal, Wuhan, China), P53 (PAb240, Cambridge, UK), P65 (E379, Abcam, Cambridge, UK), NF-κB (ab281081, Abcam, Cambridge, UK), p-NF-κB (ab279874, Abcam, Cambridge, UK), PCNA (10205-2-AP, Proteintech, Wuhan, China), Cyclin D1 (ab182858, Abcam, Cambridge, UK), PI3K (60225-1-Ig, Proteintech, Wuhan, China),
Techniques: CCK-8 Assay, Proliferation Assay, Western Blot, Expressing, Control, Immunofluorescence, Staining